BamHI RFLP at the GLUT3 locus

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BamHI RFLP at the insulin-like growth factor II (IGF2) locus on chromosome 11.

SOURCE/DESCRIPTION: phins311 (vector-pBR322) contains an 8.6 kbp EcoRI fragment from the insulin (INS)/IGF2 genomic clone lambda hINS-3 (Bell et al 1985). This fragment contains an exon encoding part of the 5' untranslated region of IGF-II mRNA as well as adjacent intron sequences. POLYMORPHISM: BamHI digestion reveals invariant fragments of 17.0, 2.1, 1.8 and 1.0 kbp and polymorphic fragments ...

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Asp700 RFLP at the D8S45 locus.

Source/Description of Clone: Clone pALP13-8 is a single copy 750 bp EcoRV fragment, 0.9 kb 3' of exon 4, subcloned from a 6.6 kb BamHI fragment containing the complete human c-fos oncogene (FOS) (1). Polymorphisms: are detected with TaqI (A alleles) and BstNI (B alleles) (see Figure). A) TaqI: Presence of a 1.6 kb fragment or its allele, a 0.6 kb fragment. B) BstNI: Presence of an 0.85 kb fragm...

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A BstE II RFLP at the DXS153 locus.

Source/Description: cpX12 is a 1.1 kb EcoRI single-copy genomic fragment cloned into pAT153 from a pool of 100 cosmids isolated from a human/hamster somatic cell hybrid containing only the human X chromosome (1). Polymorphism: cpX12 reveals a two-allele restriction fragment length polymorphism with bands at 0.7 kb and 1.9 kb (Al) or 2.6 kb (A2) after Southern blot analysis of human genomic DNA ...

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Source/Description: A 3.6 kb EcoRI fragment (lambda ABL3) containing portions of exons 7 and 8 and the intervening intron of the ABL oncogene was isolated from a chromosome 9-specific library (ATCC # LA09NS01) using a 1.7 kb Abelson murine leukemia virus probe (v-abl, ONCOR, Inc.). A single copy 1.8 kb BglII fragment (ABL3) was derived from the original lambda clone and detects the StuI RFLP. P...

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BamHI RFLP of the inhibin beta B (INHBB) chain gene on chromosome 2.

Source and Description of Clone: pBT A528 contains 920 bp of the inhibin {3B genomic sequence inserted into pBR322 (1). Polymorphism: BamHI identifies two alleles: Allele AI-4.4 kb, Allele A2-3.4 kb.

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ژورنال

عنوان ژورنال: Nucleic Acids Research

سال: 1990

ISSN: 0305-1048,1362-4962

DOI: 10.1093/nar/18.16.4967